PRPF31 Antibody

SKU: orb1257362

Description

PRPF31 Antibody

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PRPF31 (NP_056444.3).
• Target: PRPF31
• Molecular Weight: Observed: 56kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

pre-mRNA processing factor 31 homolog, PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae), Protein 61K, pre-mRNA processing factor 31 homolog (yeast), DKFZp566J153, NY-BR-99, PRP31, RP11, PRP31 pre-mRNA processing factor 31 homolog (yeast), PRPF31

Western blot analysis of extracts of various cell lines, using PRPF31 antibody (orb1257362) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat heart using PRPF31 antibody (orb1257362) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using PRPF31 antibody (orb1257362) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using PRPF31 antibody (orb1257362) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

PRPF31

UniProt

Q8WWY3