Protocadherin Gamma (pan) Antibody: APC

• Catalog Number: orb150758
• Category: Antibodies
• Description: Mouse monoclonal to Protocadherin Gamma (APC). The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes..
• Target: Protocadherin Gamma
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: APC
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Purification: Protein G Purified
• Immunogen: Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941). 99% identity with human (123/124 amino acids).
• UniProt ID: Q91XZ0
• MW: 100kDa
• Tested applications: ICC, IF, IHC
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Application notes: 1 µg/ml of SMC-454 was sufficient for detection of Protocadherin gamma (pan) in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Clone Number: N159/5 (Formerly sold as S159-5)
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: PCDH gamma A antibody, PCDH-gamma-A antibody, PCDH
• Research Area: Cell Biology, Disease Biomarkers, Neuroscience
• Note: For research use only
• Entrez: 93709
• NCBI: NP_291062.1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody, Clone N159/5. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Protocadherin Gamma (pan) Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody, Clone N159/5. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Protocadherin Gamma (pan) Antibody. (D) Composite.

Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa Protocadherin Gamma protein using Mouse Anti-Protocadherin Gamma Monoclonal Antibody, Clone N159/5. Load: 10 μg. Primary Antibody: Mouse Anti-Protocadherin Gamma Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.