Prothrombin rabbit pAb

• Catalog Number: orb766731
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Prothrombin
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: Synthesized peptide derived from the Internal region of human Prothrombin. AA range 420-470
• MW: 70
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-F2 antibody, anti-Prothrombin antibody, anti-
• Note: For research use only

Western Blot analysis of Jurkat cells using Prothrombin Polyclonal Antibody. Secondary antibody was diluted at 1:20000.

Immunohistochemical analysis of paraffin-embedded rat-liver, antibody was diluted at 1:100.

Immunohistochemical analysis of paraffin-embedded mouse-liver, antibody was diluted at 1:100.

Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).