PRKCB Antibody

SKU: orb345618

Description

Protein Kinase C Beta antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:75,000 - 1:80,000, WB: 1:1,000 - 1:2,000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 76 kDa in size corresponding to PKC beta by western blotting in the appropriate cell lysate or extract. Lysates from U251or BxPC3 cells are suggested for western blotting.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Protein kinase C beta.
• Target: PRKCB
• Purity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human PKC beta protein. A BLAST analysis was used to suggest cross-reactivity with PKC beta from human and monkey sources based on a 100% homology with the immunizing sequence. Expect partial cross reactivity to PKC beta from rabbit and bovine (93% homology) and rat and mouse (87% homology) sources. Cross-reactivity with PKC beta from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Protein kinase C beta antibody, PKC ß, Protein kinase C beta type, PRKCB, PKCB, PRKCB1, PKC-beta, PKC-B

Western blot using Biorbyt's affinity purified anti-PKC beta antibody shows detection of PKC beta in ~25 µg of U251 whole cell lysate (glioma derived). A 4-12% gel was used for separation. The arrowhead corresponds to 76 kDa PKC beta. The membrane was probed with the primary antibody at a 1:1000 dilution in 5% BSA in TTBS at 4°C, overnight.

Quick Database Links

Gene Symbol

PRKCB

UniProt

P05771

RefSeq:47157322