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Phospho-PRKCB (Thr642) Rabbit Polyclonal Antibody

Catalog Number: orb6754

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb6754
CategoryAntibodies
DescriptionPhospho-PRKCB (Thr642) Rabbit Polyclonal Antibody
TargetPRKCB
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Predicted ReactivityMouse, Rat
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human PRKCZ around the phosphorylation site of Ser642 EL(p-T)PT
UniProt IDP05771
RRIDAB_10917358
MW74 kDa
Tested applicationsFC, IF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesPRKCB(phospho T642); rotein Kinase C; AAG6; Aging
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NoteFor research use only
Phospho-PRKCB (Thr642) Rabbit Polyclonal Antibody

Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-phospho-PRKCB (Thr642) antibody (orb6754), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-PRKCB (Thr642) Rabbit Polyclonal Antibody

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-phospho-PRKCB (Thr642) antibody (orb6754), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-PRKCB (Thr642) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-PKC beta 2 (Ser660)) Polyclonal Antibody, Unconjugated (orb6754) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-PRKCB (Thr642) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-PKC beta 2 (Ser660)) Polyclonal Antibody, Unconjugated (orb6754) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-PRKCB (Thr642) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-PKC beta 2 (Ser660)) Polyclonal Antibody, Unconjugated (orb6754) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-PRKCB (Thr642) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-PKC beta 2 (Ser660)) Polyclonal Antibody, Unconjugated (orb6754) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-PRKCB (Thr642) Rabbit Polyclonal Antibody

Tissue/Cell: human prostate tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-PRKCB (Ser642) Polyclonal Antibody, Unconjugated (orb6754) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Phospho-PRKCB (Thr642) Rabbit Polyclonal Antibody

Tissue/Cell: rat stomach tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-PRKCB (Ser642) Polyclonal Antibody, Unconjugated (orb6754) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.