PKC beta1/2 Antibody (Center)

SKU: orb1929645

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P - 1:25
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD3227
• Immunogen: This PKC beta1/2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-334 amino acids from the Central region of human PKC beta1/2. Antigen Region: 303-334 aa.
• Target: PRKCB
• Molecular Weight: 76869 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Protein kinase C beta type, PKC-B, PKC-beta, PRKCB, PKCB, PRKCB1

Staining PKC beta1/2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Western blot analysis of PRKCB (arrow) using PKC beta1/2 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCB gene (Lane 2).

All lanes: Anti-PKC beta1/2 Antibody (Center) at 1:2000 dilution. Lane 1: human brain lysate. Lane 2: mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 77 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PKC beta1/2 Antibody (Center) at 1:2000 dilution. Lane 1: mouse brain lysate. Lane 2: SH-SY5Y whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 77 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PKC beta1/2 Antibody (Center) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: human brain lysate. Lane 3: mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 77 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

PRKCB

UniProt

P05771

RefSeq (Protein):NP_997700.1, NP_002729.2