Protein G Antibody Fluorescein Conjugated

• Catalog Number: orb344717
• Category: Antibodies
• Description: Protein G antibody (FITC)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: FITC
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: Anti-Protein G Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and Protein G.
• Immunogen: Protein G (Streptococcus species)
• Tested applications: DOT, IF
• Dilution range: IF: 1:50 - 1:250
• Application notes: FITC Conjugated Anti-Protein G antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Antibody Type: Primary Antibody
• Storage: Store Protein G conjugated antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: rabbit anti-Protein G Antibody FITC Conjugation, F
• Note: For research use only

Dot Blot of Protein G Antibody Fluorescein Conjugated. Antigen 1: Human IgG. Antigen 2: Rat IgG. Antigen 3: Dog IgG. Load: Starting dot is 200 ng followed by a 3-fold serial dilution. Primary antibody: Protein G at 1:1000 for 60 min RT. Secondary antibody: Fluorescein rabbit anti-Protein G at 1:1000 for 30 min at RT. Block: orb348637 for 30 min at RT.