Protein A Antibody Fluorescein Conjugated

SKU: orb2652731

Description

Protein A Antibody Fluorescein Conjugated

Images & Validation

• Tested Applications: DOT, IF, WB
• Dilution Range: IF: 1:500-1:2,500
• Application Notes:
  Anti-Protein A Fluorescein is tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. 3.6 moles Fluorescein (FITC) per mole. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 2.0 mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Protein A (Staphylococcus aureus)
• Purity: Anti-Protein A Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, and purified and partially purified Protein A. Cross reactivity against Protein A from other sources is unknown.
• Conjugation: FITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Thimerosal. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Protein A Antibody, FITC Conjugated goat anti-Protein A Antibody, Protein A Fluorescein, Protein A FITC Antibody

Western Blot of Goat anti-Protein A Antibody. Lane 1: Protein A Reduced. Lane 2: Protein A Reduced. Lane 3: Protein A Non-Reduced. Lane 4: Protein A Non-Reduced. Load: Lane 1 and 3 - 1.0 µg per lane, Lane 2 and 4 - 0.25 ug per lane. Primary antibody: Protein A antibody at 1:5000 for overnight at 4°C. Secondary antibody: DyLight™ 649 goat secondary antibody at 1:10000 for 90 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~50 kDa for Protein A. Other band(s): Protein A splice variants and isoforms.