Prostein antibody (MSVA-460R)

• Catalog Number: orb2276240
• Category: Antibodies
• Description: Prostein antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: Biorbyt-460R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG
• Immunogen: Synthesized peptides to the N terminus of human SLC45A3 protein
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: Q96JT2
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: SLC45A3, PCANAP6, Prostate Cancer Associated Prote
• Note: For research use only
• Application notes: Positive Control: Stomach mucosa: At least a moderate, granular, cytoplasmic prostein positivity should be seen in surface epithelial cells. Negative Control: Stomach mucosa: Prostein immunostaining should be absent in gastric glands. Cellular Localization: Membranous Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Normal prostate with strong granular perinuclear cytoplasmic prostein staining of luminal cells.

Prostatic adenocarcinoma Gleason 336 with strong granular perinuclear cytoplasmic prostein staining of tumor cells.

Stomach mucosa with granular cytoplasmic prostein staining of surface epithelial cells.