Prolactin Antibody / PRL

• Catalog Number: orb607118
• Category: Antibodies
• Description: Prolactin is a growth factor that is secreted by the anterior pituitary. It is necessary for the proliferation and differentiation of the mammary glands. Prolactin is useful in the classification of pituitary tumors and study of pituitary disease. It also plays a role in the development of mammary cancer, functioning dually as a mitogen and a differentiating agent.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PRL/2910
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A recombinant human partial protein corresponding to amino acids 63-201 was used as the immunogen for the Prolactin antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Prolactin antibody is available for research use only.
• UniProt ID: P01236
• Storage: Store the Prolactin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Prolactin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human U937 cell lysate with Prolactin antibody (clone (PRL/2910). Predicted molecular weight: ~25 kDa.

SDS-PAGE analysis of purified, BSA-free Prolactin antibody (clone PRL/2910) as confirmation of integrity and purity.

IHC testing of FFPE human pituitary tissue with Prolactin antibody (clone PRL/2910). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.