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PROKR2 Antibody

SKU: orb1249435

Description

PROKR2 Antibody

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 45kDa band observed in Human Brain (Cerebellum), Testis and Thyroid Gland and in Rat Brain, Stomach and Testis lysates (calculated MW of 44.0kDa according to Human NP_658986.1 and 47.9kDa according to Rat NP_620434.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-NGNTSFTPNFN
TargetPROKR2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
DisclaimerFor research use only

Alternative Names

PROKR2, prokineticin receptor 2, GPR73L1, GPR73b, GPRg2, KAL3, PKR2, dJ680N4.3, G protein-coupled receptor 73-like 1, OTTHUMP00000030192

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PROKR2 Antibody

orb1249435 (1 ug/ml) staining of Rat Stomach lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

PROKR2 Antibody

orb1249435 (0.1 ug/ml) staining of Human Cerebellum (A), Rat Brain (B) and Rat Testis (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

PROKR2 Antibody

orb1249435 (3.75 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

PROKR2 Antibody (orb1249435)

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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