PROKR2 Antibody

SKU: orb1249435

Description

PROKR2 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 45kDa band observed in Human Brain (Cerebellum), Testis and Thyroid Gland and in Rat Brain, Stomach and Testis lysates (calculated MW of 44.0kDa according to Human NP_658986.1 and 47.9kDa according to Rat NP_620434.1). Recommended concentration: 0.3-1µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-NGNTSFTPNFN
• Target: PROKR2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PROKR2, prokineticin receptor 2, GPR73L1, GPR73b, GPRg2, KAL3, PKR2, dJ680N4.3, G protein-coupled receptor 73-like 1, OTTHUMP00000030192

orb1249435 (1 ug/ml) staining of Rat Stomach lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249435 (0.1 ug/ml) staining of Human Cerebellum (A), Rat Brain (B) and Rat Testis (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249435 (3.75 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

PROKR2

UniProt

Q8NFJ6

RefSeq (Protein):NP_658986.1