Prohibitin (PHB1) Antibody (N-term)

• Catalog Number: orb1931544
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This Prohibitin (PHB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the N-terminal region of human Prohibitin (PHB1).
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P35232
• MW: 29804 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:2000, IHC-P: 1:100, IHC-P: 1:100, FC: 1:10~50
• Clone Number: RB10625
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Prohibitin, PHB
• Note: For research use only
• NCBI: NP_001268425.1, NP_002625.1, NP_001268644.1

Flow cytometric analysis of ATDC5 cells using PHB1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemical analysis on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-Prohibitin (PHB1) Antibody (N-term) at 1:2000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: A431 whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: Hela whole cell lysate. Lane 6: Mouse kidney tissue lysate. Lane 7: NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 30 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.