Proenkephalin Antibody

• Catalog Number: orb1247069
• Category: Antibodies
• Description: Proenkephalin Antibody
• Target: PENK
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-RSHHQDGSDNEE
• UniProt ID: P01210
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37kDa band observed in Human Adrenal Gland lysates (calculated MW of 30.8kDa according to NP_006202.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Normant and Loh, Endocrinology. 1998 Apr;139(4):2137-45; PMID: 9529003). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Frozen section of Human Hypothalamus shows staining of dense enkephalinergic axon plexus and scattered neuronal cell bodies (higher magnification inset) in the human infundibular nucleus. Recommended concentration: 0.3-1ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: proenkephalin, PENK
• Note: For research use only
• NCBI: NP_006202.1

orb1247069 (0.3 ug/ml) staining of Human Adrenal Gland lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247069 (0.3 ug/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, Cy3-staining.