PRODH Antibody

• Catalog Number: orb1247070
• Category: Antibodies
• Description: PRODH Antibody
• Target: PRODH
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-ERDGSGTNKRDKQYQ
• UniProt ID: O43272
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:This antibody has been successfully used in westen blot on MCF-7 cells: Zareba et al, Oncotarget. 2018 Feb 9;9(17):13748-13757, PMID: 29568391.Immunohistochemistry:In paraffin embedded Cerebellum shows mitochondrial staining in smaller neurons. Paraffin embedded Human Liver and Colon. Recommended concentration: 3.75ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PRODH, proline dehydrogenase (oxidase) 1 , HSPOX2,
• Note: For research use only
• NCBI: NP_057419.2

orb1247070 (3.75 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247070 (3.75 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247070 (3.75 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.