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PRODH Antibody

SKU: orb1247070

Description

PRODH Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Predicted ReactivityMouse
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:This antibody has been successfully used in westen blot on MCF-7 cells: Zareba et al, Oncotarget. 2018 Feb 9;9(17):13748-13757, PMID: 29568391.Immunohistochemistry:In paraffin embedded Cerebellum shows mitochondrial staining in smaller neurons. Paraffin embedded Human Liver and Colon. Recommended concentration: 3.75µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-ERDGSGTNKRDKQYQ
TargetPRODH
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

PRODH, proline dehydrogenase (oxidase) 1 , HSPOX2, PIG6, PRODH1, PRODH2, SCZD4, TP53I6, FLJ33744, MGC148078, MGC148079, Proline oxidase, mitochondrial, p53 induced protein, proline dehydrogenase (proline oxidase ), proline oxidase 2, tumor protein p53 inducible protein 6

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PRODH Antibody

orb1247070 (3.75 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

PRODH Antibody

orb1247070 (3.75 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

PRODH Antibody

orb1247070 (3.75 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

PRODH Antibody (orb1247070)

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Available Sizes

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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