Procollagen Type III, human, bovine Antibody

• Catalog Number: orb867272
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Procollagen Type III
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Concentration: app. 1 mg/ml
• Purification: affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
• Immunogen: Procollagen type III deprived of the C-terminal PIIICP peptide
• UniProt ID: P02461
• Tested applications: ELISA, IF, IHC-P, RIA, WB
• Dilution range: (purified, lyophilized) IFA ? 1:40, IHC(P) ? 1:500, ELISA 1:100 - 1:200 (OD ? 500), RIA >1:200
• Application notes: Positive Control: Human or bovine skin and liver. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC(P) 60 min at RT or 2-8°C over night
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Cell Biology
• Note: For research use only