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Procollagen Type III, human, bovine
Catalog Number: orb867272
| Catalog Number | orb867272 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Procollagen Type III |
| Species/Host | Rabbit |
| Tested applications | ELISA, IF, IHC-P, RIA, WB |
| Immunogen | Procollagen type III deprived of the C-terminal PIIICP peptide |
| Antibody Type | Primary Antibody |
| Concentration | app. 1 mg/ml |
| Dilution range | (purified, lyophilized) IFA ? 1:40, IHC(P) ? 1:500, ELISA 1:100 - 1:200 (OD ? 500), RIA >1:200 |
| Conjugation | Unconjugated |
| Hazard Information | *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. |
| UniProt ID | P02461 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | affinity purified antibody lyophilized from phosphate buffered solution; no rAlbumin and preservative added! |
| Note | For research use only |
| Application notes | Positive Control: Human or bovine skin and liver. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC(P) 60 min at RT or 2-8°C over night |
| Expiration Date | 12 months from date of receipt. |
