Pro-glucagon Rabbit pAb Discontinued

SKU: orb539598

Description

Rabbit Polyclonal antibody to GLP1

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution Range: WB: 1:500-1:2000, IHC-P: 1:50-1:200, IF: 1:50-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-89 of human Pro-glucagon (NP_002045.1).
• Molecular Weight: 21kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GCG, GLP-1, GLP1, GLP2, GRPP, glucagon

Immunofluorescence analysis of paraffin-embedded mouse pancreas using Pro-glucagon Rabbit pAb (orb539598) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of paraffin-embedded rat pancreas using Pro-glucagon Rabbit pAb (orb539598) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry analysis of paraffin-embedded Human islet using Pro-glucagon Rabbit pAb (orb539598) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse pancreatic islet using Pro-glucagon Rabbit pAb (orb539598) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat pancreatic islet using Pro-glucagon Rabbit pAb (orb539598) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Western blot analysis of various lysates using Pro-glucagon Rabbit pAb (orb539598) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Quick Database Links

UniProt

P01275

Nader Goodarzi et al. Histology and immunofluorescent study of the pancreas in lovebird (Agapornis personatus) Vet Med Sci ., 0(2), e1394 (2024)

PubMed: 38459816

Applications

IF

Reactivity

Other