Pro-EGF Antibody

• Catalog Number: orb1248193
• Category: Antibodies
• Description: Pro-EGF Antibody
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: SRQERVCNIEKNVS
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 160kDa
• Target: Pro-EGF
• UniProt ID: P01133
• NCBI: NP_001171601.1, NP_001171602.1, NP_001954.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: EGF, epidermal growth factor, HOMG4, URG, OTTHUMP0
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 160kDa double band observed in Human Prostate lysates (calculated MW of 134kDa according to NP_001954.2). The two bands represent glycosylated forms of the mature protein. Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skin. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248193 (1 ug/ml) staining of Human Prostate lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248193 (5 ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.