Pro-EGF Antibody

SKU: orb1248193

Description

Pro-EGF Antibody

Research Area

Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 160kDa double band observed in Human Prostate lysates (calculated MW of 134kDa according to NP_001954.2). The two bands represent glycosylated forms of the mature protein. Recommended concentration: 1-3µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skin. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: SRQERVCNIEKNVS
• Target: Pro-EGF
• Molecular Weight: Approx 160kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EGF, epidermal growth factor, HOMG4, URG, OTTHUMP00000162922, OTTHUMP00000219721, OTTHUMP00000219722, beta-urogastrone, pro-epidermal growth factor

orb1248193 (1 ug/ml) staining of Human Prostate lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248193 (5 ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

Pro-EGF

UniProt

P01133

RefSeq (Protein):NP_001954.2, NP_001171601.1, NP_001171602.1