PRMT5 Antibody

• Catalog Number: orb1259624
• Category: Antibodies
• Description: PRMT5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 40-140 of human PRMT5 (NP_006100.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 70kDa
• Target: PRMT5
• UniProt ID: O14744
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: PRMT5, JBP1, SKB1, IBP72, SKB1Hs, HRMT1L5
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PRMT5 antibody (orb1259624) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Immunohistochemistry of paraffin-embedded rat brain using PRMT5 antibody (orb1259624) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using PRMT5 antibody (orb1259624) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using PRMT5 antibody (orb1259624) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using PRMT5 antibody (orb1259624). Blue: DAPI for nuclear staining.