PRMT5 Antibody (N-term)

SKU: orb1936412

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Bovine, Monkey

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD55006
• Immunogen: This PRMT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-104 amino acids from the N-terminal region of human PRMT5. Antigen Region: 76-104 aa.
• Target: PRMT5
• Molecular Weight: 72684 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Protein arginine N-methyltransferase 5, 211-, 72 kDa ICln-binding protein, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Shk1 kinase-binding protein 1 homolog, SKB1 homolog, SKB1Hs, Protein arginine N-methyltransferase 5, N-terminally processed, PRMT5, HRMT1L5, IBP72, JBP1, SKB1

All lanes: Anti-PRMT5 Antibody (N-term) at 1:1000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 73 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

PRMT5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PRMT5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

PRMT5

UniProt

O14744

RefSeq (Protein):NP_001034708.1, NP_006100.2