PRKDC Antibody

SKU: orb1256238

Description

PRKDC Antibody

Research Area

Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide of human PRKDC
• Target: PRKDC
• Molecular Weight: Observed: 469kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PRKDC, DNA-PK, DNAPK, DNPK1, HYRC, HYRC1, p350, Scid, XRCC7, DNA-PK catalytic subunit, DNA-PKcs, p460, DNA-dependent protein kinase catalytic subunit

Western blot analysis of extracts of various cell lines, using PRKDC antibody (orb1256238) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PRKDC antibody (orb1256238) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using PRKDC antibody (orb1256238) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using PRKDC antibody (orb1256238) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U-2OS cells using PRKDC antibody (orb1256238) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

PRKDC

UniProt

P78527