PRKDC Antibody

• Catalog Number: orb1256238
• Category: Antibodies
• Description: PRKDC Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: A synthetic peptide of human PRKDC
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 469kDa
• Target: PRKDC
• UniProt ID: P78527
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: PRKDC, DNA-PK, DNAPK, DNPK1, HYRC, HYRC1, p350, Sc
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PRKDC antibody (orb1256238) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PRKDC antibody (orb1256238) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using PRKDC antibody (orb1256238) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using PRKDC antibody (orb1256238) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U-2OS cells using PRKDC antibody (orb1256238) at dilution of 1:100. Blue: DAPI for nuclear staining.