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PRG4 Antibody (N-Term)
Catalog Number: orb1925385
| Catalog Number | orb1925385 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This PRG4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 176-210 amino acids from the human region of human PRG4. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| UniProt ID | Q92954 |
| MW | 151061 Da |
| Tested applications | IF, WB |
| Dilution range | IF: 1:25, WB: 1:500 |
| Antibody Type | Primary Antibody |
| Clone Number | RB57887 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Proteoglycan 4, Lubricin, Megakaryocyte-stimulatin Read more... |
| Note | For research use only |
Anti-PRG4 Antibody (N-Term) at 1:500 dilution + Human liver whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 151 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (human cervical epithelial adenocarcinoma cell line) cells labeling PRG4 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on Hela cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
