PREX1 Antibody (C-term)

• Catalog Number: orb1937358
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB31307
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:2000, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:10~50, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 186203 Da
• Target: This PREX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1134-1163 amino acids from the C-terminal region of human PREX1.
• UniProt ID: Q8TCU6
• NCBI: NP_065871.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Phosphatidylinositol 3,5-trisphosphate-dependent R
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-PREX1 Antibody (C-term) at 1:2000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: SK-BR-3 whole cell lysate. Lane 3: THP-1 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 186 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining PREX1 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

Overlay histogram showing U-2OS cells (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

PREX1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PREX1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

PREX1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35 ug/lane). This demonstrates the PREX1 antibody detected the PREX1 protein (arrow).