Presenilin 1 Antibody (C-term) [Knockout Validated]

• Catalog Number: orb1787818
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: 330-359 aa
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:500-1:2000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 52668 Da
• Target: This Presenilin 1 (PSEN1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 330-359 amino acids from the C-terminal region of human Presenilin 1 (PSEN1).
• UniProt ID: P49768
• NCBI: NP_000012.1, NP_015557.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Presenilin-1, PS-1, 3423-, Protein S182, Presenili
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Confocal immunofluorescent analysis of Presenilin 1 (PSEN1) Antibody (C-term) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Presenilin 1 (PSEN1) Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

All lanes: Anti-PSEN1 Antibody (C-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HL-60 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 53 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

A dominant 59.6 kDa band and faint 87.6 kDa band for both the Hela wild type and knock out lysates were observed (3 ug/ml anti-PSEN1) vs the predicted size of 52.7 kDa. The molecular weight discrepancy could be due a post-translationally modified form of the target protein, a splice-variant form of the target protein, or an unrelated protein which shares the antibody-reactive epitope. The presence of bands in the knock out lysates suggest incomplete knockout of the target gene.