TTR Antibody

SKU: orb1247074

Description

TTR Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: EIA, ELISA, IHC
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Preliminary experiments gave an approx 20-22kDa band in Human Heart, Kidney, Liver, Lung, Placenta and Testis lysates after 1µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 15.9kDa according to NP_000362.1. The 20-22kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Pancreas. Recommended concentration: 10µg/mL.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant TTR captured by a rabbit antibody. Recommended reporter concentration: 5-10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-YKVEIDTKSYWK
• Target: TTR
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TTR, transthyretin (prealbumin, amyloidosis type I), HGNC:12405, HsT2651, PALB, TBPA, prealbumin, transthyretin

orb1247074 (5 ug/ml) as the reporter antibody.

orb1247074 (10 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

TTR

UniProt

P02766

RefSeq (Protein):NP_000362.1