PRDM2 (RIZ1) Antibody

• Catalog Number: orb1939437
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 33AT1045
• Tested applications: ChIP, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1
• Antibody Type: Primary Antibody
• Dilution range: CHIP: 1:100, CHIP: 1:100, CHIP: 1:100, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 188915 Da
• Target: Purified recombinant GST fusion protein encoding aa 1-347 of human RIZ1. This antibody is specific for RIZ1, it does not recognize the isoform beta (RIZ2).
• UniProt ID: Q13029
• NCBI: NP_056950.2, NP_001007258.1, NP_001129082.1, NP_036363.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: PR domain zinc finger protein 2, GATA-3-binding pr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ChIP analysis of estrogen target genes. (A) Soluble chromatin was prepared from MCF7 cells not treated or treated with E2 for 45 min. Immunoprecipitation was performed with antibody against RIZ1. DNA extractions were amplified by using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter.

ChIP analysis of estrogen target genes. (B) Time course analysis of RIZ1 binding to the pS2 gene promoter. MCF7 cells treated with E2 for different periods of time, as indicated at the top of each lane, were processed for ChIP analysis. Immunoprecipitation was performed with antibody to RIZ1 and control immunoglobulin G as indicated.

ChIP analysis of estrogen target genes. (C) ChIP analysis was performed on RIZ1 knockout mouse embryonic fibroblasts by using anti-RIZ1 Antibody. Immunoprecipitated chromatin DNA was analyzed by PCR with primers in the G6pd promoter region and in the cdc25c promoter region. Adapted from Fig 6 in Carling et al., 2004 (see citation 2).

Western blot analysis of anti-PRDM2 Mab in lysate from PRDM2 transgenic lysate. Secondary HRP-anti-mouse was used for signal visualization with chemiluminescence.