PRDM2 (RIZ1) Antibody

• Catalog Number: orb1939437
• Category: Antibodies
• Description: PRDM2 (RIZ1) Antibody
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• UniProt ID: Q13029
• MW: 188915 Da
• Tested applications: ChIP, WB
• Dilution range: WB - 1:1000, CHIP - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: KMT8, RIZ
• Research Area: Cancer Biology, Neuroscience
• Note: For research use only

ChIP analysis of estrogen target genes. (A) Soluble chromatin was prepared from MCF7 cells not treated or treated with E2 for 45 min. Immunoprecipitation was performed with antibody against RIZ1. DNA extractions were amplified by using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter.

ChIP analysis of estrogen target genes. (B) Time course analysis of RIZ1 binding to the pS2 gene promoter. MCF7 cells treated with E2 for different periods of time, as indicated at the top of each lane, were processed for ChIP analysis. Immunoprecipitation was performed with antibody to RIZ1 and control immunoglobulin G as indicated.

ChIP analysis of estrogen target genes. (C) ChIP analysis was performed on RIZ1 knockout mouse embryonic fibroblasts by using anti-RIZ1 Antibody. Immunoprecipitated chromatin DNA was analyzed by PCR with primers in the G6pd promoter region and in the cdc25c promoter region. Adapted from Fig 6 in Carling et al., 2004 (see citation 2).

Western blot analysis of anti-PRDM2 Mab in lysate from PRDM2 transgenic lysate. Secondary HRP-anti-mouse was used for signal visualization with chemiluminescence.