PRDM11 Antibody

• Catalog Number: orb1247077
• Category: Antibodies
• Description: PRDM11 Antibody
• Target: PRDM11
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: LSEGRVRSGLCGG
• UniProt ID: Q9NQV5
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human Lung Carcinoma A549 cell line lysates (calculated MW of 58kDa according to NP_064614.2). Recommended concentration: 2-3ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PRDM11, PR domain containing 11, PR-domain contain
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_064614.2

orb1247077 (2 ug/ml) staining of A549 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247077 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.