PRAMEF3 Antibody (C-term)

• Catalog Number: orb30683
• Category: Antibodies
• Description: Peptide affinity purified rabbit polyclonal antibody.
• Target: This PRAMEF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 420-449 amino acids from the C-terminal region of human PRAMEF3.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: Synthetic Peptide
• UniProt ID: Q5TYW8
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:10-50
• Antibody Type: Primary Antibody
• Clone Number: RB31002
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-PRAME family member 3 antibody, anti-PRAMEF3
• Research Area: Cell Biology
• Note: For research use only

PRAMEF3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human melanoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PRAMEF3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of PRAMEF3 Antibody (C-term) Pab pre-incubated without (lane 1) and with (lane 2) blocking peptide in NCI-H460 cell line lysate. PRAMEF3 Antibody (C-term) (arrow) was detected using the purified Pab.