PRAME Recombinant Rabbit Monoclonal Antibody

SKU: orb1499288

Description

PRAME Recombinant Rabbit Monoclonal Antibody

Research Area

Cancer Research, Coactivators and Corepressors, DNA and RNA Research, Tumor Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-1000
• Reactivity: Human
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: R1D8
• Immunogen: KLH conjugated synthetic peptide derived from human PRAME
• Target: PRAME
• Molecular Weight: 56 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MAPE; Melanoma antigen preferentially expressed in tumors; OIP 4; OIP-4; OIP4; OPA interacting protein 4; Opa interacting protein OIP4; OPA-interacting protein 4; PRAME_HUMAN; Preferentially expressed antigen in melanoma; Preferentially expressed antigen of melanoma; RP23-250F8.3.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 4°C, overnight, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: Ramos, 2: K562, 3: HeLa, 4: A375, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 58 kDa, Observed MW: 58 kDa.

Quick Database Links

Gene Symbol

PRAME

UniProt

P78395