PRAK (MAPKAPK5) Antibody (T182)

SKU: orb1929472

Description

Research Area

Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution Range	WB - 1:1000
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	54220 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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PRAK

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Western blot analysis of MAPKAPK5 (arrow) using rabbit polyclonal MAPKAPK5 Antibody (T182). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAPKAPK5 gene.

Anti-MAPKAPK5 Antibody (T182) at 1:1000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

Q8IW41

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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