PPP4R1 Antibody

• Catalog Number: orb1255885
• Category: Antibodies
• Description: PPP4R1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 324-647 of human PPP4R1 (NP_001035847.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 130kDa
• Target: PPP4R1
• UniProt ID: Q8TF05
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: MEG1, PP4(Rmeg), PP4R1, serine/threonine-protein p
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PPP4R1 antibody (orb1255885) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded human liver using PPP4R1 antibody (orb1255885) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using PPP4R1 antibody (orb1255885) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200 ug extracts of 293T cells, using 3 ug PPP4R1 antibody (orb1255885). Western blot was performed from the immunoprecipitate using PPP4R1 antibody (orb1255885) at a dilution of 1:1000.