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Catalog Number | orb1927352 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB43624 |
Tested applications | IF, IHC-P, WB |
Predicted Reactivity | Mouse, Porcine, Rabbit, Rat |
Reactivity | Human |
Isotype | Rabbit IgG |
Dilution range | IF: 1:25, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 51692 Da |
Target | This PPP2R2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-71 amino acids from the N-terminal region of human PPP2R2A. |
UniProt ID | P63151 |
NCBI | NP_001171062.1, NP_002708.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Serine/threonine-protein phosphatase 2A 55 kDa reg Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
PPP2R2A Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35 ug/lane).This demonstrates the PPP2R2A antibody detected the PPP2R2A protein (arrow).
Immunohistochemical analysis of paraffin-embedded H. liver section using PPP2R2A Antibody (N-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. kidney section using PPP2R2A Antibody (N-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Fluorescent image of Hela cells stained with PPP2R2A Antibody (N-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |