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Item 1 of 2
PPM1H Antibody: PerCP
Catalog Number: orb1713438
| Catalog Number | orb1713438 |
|---|---|
| Category | Antibodies |
| Description | Human Anti-Human PPM1H Monoclonal IgG1 |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | 10D2 |
| Tested applications | ELISA, ICC, IF, IHC, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG1 |
| Immunogen | Recombinant Human PPM1H Protein |
| Concentration | 1mg/ml |
| Dilution range | WB (1:1000), ICC/IF (1:500); optimal dilutions for assays should be determined by the user. |
| Conjugation | PerCP |
| Target | PPM1H |
| UniProt ID | Q9ULR3 |
| Storage | -20°C |
| Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
| Alternative names | Protein phosphatase Mg2+/Mn2+ dependent 1H Antibod Read more... |
| Note | For research use only |
| Application notes | A 1:1000 dilution of SMC-616 was sufficient for detection of PPM1H in 10 µg of Hu, Ms, Rt Kidney Cells (HEK293), Alveolar Basal Epithelial cells (A549) by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PPM1H Monoclonal Antibody, Clone 10D2. Tissue: Alveolar Basal Epithelial cells (A549). Species: Hu, Ms, Rt. Primary Antibody: Mouse Anti-PPM1H Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Alexa Fluor 594 Donkey anti mouse secondary diluted in 0.2% BSA + 0.02% sodium azide in PBS at 1:1000 for 1 hour at RT. Counterstain: Cells mounted and co-stained with DAPI using ProLong Gold antifade reagent with DAPI (Invitrogen). Localization: Intracellular, Nucleoplasm. Magnification: 63x.
Western Blot analysis of Hu, Ms, Rt Kidney Cells (HEK293), Alveolar Basal Epithelial cells (A549) showing detection of ~55 PPM1H protein using Mouse Anti-PPM1H Monoclonal Antibody, Clone 10D2. Lane 1: MW Ladder. Lane 2: A549. Lane 3: HEK293. Load: 30 ug. Block: 5% Skim Milk in TBST, 1h, RT. Primary Antibody: Mouse Anti-PPM1H Monoclonal Antibody at 1:1000 for Overnight, 4C. Secondary Antibody: Donkey anti-mouse 800 (LiCOR) at 1:25000 for 1 hour at RT. Color Development: Odyssey CLx Western Blot imaging. Predicted/Observed Size: ~55. Other Band (s): ~30, ~100.
