PPM1H Antibody (HRP)

SKU: orb1713439

Description

Mouse Anti-Human PPM1H Monoclonal IgG1

Research Area

Cancer Biology, Neuroscience, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution Range: WB (1:1000), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:1000 dilution of SMC-616 was sufficient for detection of PPM1H in 10 µg of Hu, Ms, Rt Kidney Cells (HEK293), Alveolar Basal Epithelial cells (A549) by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 10D2
• Immunogen: Recombinant Human PPM1H Protein
• Target: PPM1H
• Purification: Protein G Purified
• Conjugation: HRP

Storage & Handling

• Storage: -20°C
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Protein phosphatase Mg2+/Mn2+ dependent 1H, NERPP2C, Protein phosphatase 1H, PPM1H, KIAA1157

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PPM1H Monoclonal Antibody, Clone 10D2. Tissue: Alveolar Basal Epithelial cells (A549). Species: Hu, Ms, Rt. Primary Antibody: Mouse Anti-PPM1H Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Alexa Fluor 594 Donkey anti mouse secondary diluted in 0.2% BSA + 0.02% sodium azide in PBS at 1:1000 for 1 hour at RT. Counterstain: Cells mounted and co-stained with DAPI using ProLong Gold antifade reagent with DAPI (Invitrogen). Localization: Intracellular, Nucleoplasm. Magnification: 63x.

Western Blot analysis of Hu, Ms, Rt Kidney Cells (HEK293), Alveolar Basal Epithelial cells (A549) showing detection of ~55 PPM1H protein using Mouse Anti-PPM1H Monoclonal Antibody, Clone 10D2. Lane 1: MW Ladder. Lane 2: A549. Lane 3: HEK293. Load: 30 ug. Block: 5% Skim Milk in TBST, 1h, RT. Primary Antibody: Mouse Anti-PPM1H Monoclonal Antibody at 1:1000 for Overnight, 4C. Secondary Antibody: Donkey anti-mouse 800 (LiCOR) at 1:25000 for 1 hour at RT. Color Development: Odyssey CLx Western Blot imaging. Predicted/Observed Size: ~55. Other Band (s): ~30, ~100.

Quick Database Links

Gene Symbol

PPM1H

UniProt

Q9ULR3