PPM1H Antibody: Biotin

• Catalog Number: orb1713441
• Category: Antibodies
• Description: Human Anti-Human PPM1H Monoclonal IgG1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 10D2
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Recombinant Human PPM1H Protein
• Concentration: 1mg/ml
• Dilution range: WB (1:1000), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
• Conjugation: Biotin
• Target: PPM1H
• UniProt ID: Q9ULR3
• Storage: -20°C
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: Protein phosphatase Mg2+/Mn2+ dependent 1H Antibod
• Note: For research use only
• Application notes: A 1:1000 dilution of SMC-616 was sufficient for detection of PPM1H in 10 µg of Hu, Ms, Rt Kidney Cells (HEK293), Alveolar Basal Epithelial cells (A549) by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PPM1H Monoclonal Antibody, Clone 10D2. Tissue: Alveolar Basal Epithelial cells (A549). Species: Hu, Ms, Rt. Primary Antibody: Mouse Anti-PPM1H Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Alexa Fluor 594 Donkey anti mouse secondary diluted in 0.2% BSA + 0.02% sodium azide in PBS at 1:1000 for 1 hour at RT. Counterstain: Cells mounted and co-stained with DAPI using ProLong Gold antifade reagent with DAPI (Invitrogen). Localization: Intracellular, Nucleoplasm. Magnification: 63x.

Western Blot analysis of Hu, Ms, Rt Kidney Cells (HEK293), Alveolar Basal Epithelial cells (A549) showing detection of ~55 PPM1H protein using Mouse Anti-PPM1H Monoclonal Antibody, Clone 10D2. Lane 1: MW Ladder. Lane 2: A549. Lane 3: HEK293. Load: 30 ug. Block: 5% Skim Milk in TBST, 1h, RT. Primary Antibody: Mouse Anti-PPM1H Monoclonal Antibody at 1:1000 for Overnight, 4C. Secondary Antibody: Donkey anti-mouse 800 (LiCOR) at 1:25000 for 1 hour at RT. Color Development: Odyssey CLx Western Blot imaging. Predicted/Observed Size: ~55. Other Band (s): ~30, ~100.