PPM1H Antibody (APC)

SKU: orb1713442

This product can be custom made

Description

Mouse Anti-Human PPM1H Monoclonal IgG1

Research Area

Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	ICC, IF, WB
Dilution Range	WB (1:1000), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
Reactivity	Human, Mouse, Rat
Application Notes	A 1:1000 dilution was sufficient for detection of PPM1H in 10 µg of Hu, Ms, Rt Kidney Cells (HEK293), Alveolar Basal Epithelial cells (A549) by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	10D2
Immunogen	Recombinant Human PPM1H Protein
Target	PPM1H
Purification	Protein G Purified
Conjugation	APC

Storage & Handling

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Storage	-20°C
Buffer/Preservatives	95.46mM Phosphate, 2.48mM MES and 2mM EDTA
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Protein phosphatase Mg2+/Mn2+ dependent 1H, NERPP2C, Protein phosphatase 1H, PPM1H, KIAA1157

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PPM1H Monoclonal Antibody, Clone 10D2. Tissue: Alveolar Basal Epithelial cells (A549). Species: Hu, Ms, Rt. Primary Antibody: Mouse Anti-PPM1H Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Alexa Fluor 594 Donkey anti mouse secondary diluted in 0.2% BSA + 0.02% sodium azide in PBS at 1:1000 for 1 hour at RT. Counterstain: Cells mounted and co-stained with DAPI using ProLong Gold antifade reagent with DAPI (Invitrogen). Localization: Intracellular, Nucleoplasm. Magnification: 63x.

Western Blot analysis of Hu, Ms, Rt Kidney Cells (HEK293), Alveolar Basal Epithelial cells (A549) showing detection of ~55 PPM1H protein using Mouse Anti-PPM1H Monoclonal Antibody, Clone 10D2. Lane 1: MW Ladder. Lane 2: A549. Lane 3: HEK293. Load: 30 ug. Block: 5% Skim Milk in TBST, 1h, RT. Primary Antibody: Mouse Anti-PPM1H Monoclonal Antibody at 1:1000 for Overnight, 4C. Secondary Antibody: Donkey anti-mouse 800 (LiCOR) at 1:25000 for 1 hour at RT. Color Development: Odyssey CLx Western Blot imaging. Predicted/Observed Size: ~55. Other Band (s): ~30, ~100.