PPM1F Antibody (N-term)

SKU: orb1928484

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:50-100
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD7145
• Immunogen: This PPM1F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-40 amino acids from the N-terminal region of human PPM1F. Antigen Region: 11-40 aa.
• Target: PPM1F
• Molecular Weight: 49831 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Protein phosphatase 1F, Ca(2+)/calmodulin-dependent protein kinase phosphatase, CaM-kinase phosphatase, CaMKPase, Partner of PIX 2, Protein fem-2 homolog, hFem-2, PPM1F, KIAA0015, POPX2

Western blot analysis of anti-PPM1F Pab in mouse liver tissue lysate (35 ug/lane). PPM1F (arrow) was detected using the purified Pab.

Western blot analysis of PPM1F (arrow) using PPM1F Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PPM1F gene (Lane 2).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

PPM1F

UniProt

P49593

RefSeq (Protein):NP_055449.1