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Catalog Number | orb1262509 |
---|---|
Category | Antibodies |
Description | PPID Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC-P, WB |
Predicted Reactivity | Rat |
Reactivity | Human, Mouse |
Isotype | Rabbit Ig |
Immunogen | This PPID antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-115 amino acids from the N-terminal region of human PPID. |
Concentration | batch dependent |
Dilution range | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 41 kDa |
Target | PPID |
UniProt ID | Q08752 |
NCBI | Q08752 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Peptidyl-prolyl cis-trans isomerase D, PPIase D, 4 Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of PPID Antibody in NIH-3T3 cell line lysates (35 ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with PPID Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PPID using rabbit polyclonal PPID Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPID gene.
FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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