PP5 Antibody

SKU: orb376709

Description

Mouse Anti-Human PP5 Monoclonal IgG1

Research Area

Epigenetics & Chromatin, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human
• Application Notes:
  1 µg/ml of SMC-244 was sufficient for detection of PP5 in 15 µg of human A431 lysates by ECL analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 12F7
• Immunogen: Full length human PP5 protein
• Target: PP5
• Molecular Weight: 58kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PPP5, PPT, Protein phosphatase T, Serine/threonine protein phosphatase 5, PPP5C

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PP5 Monoclonal Antibody, Clone 12F7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody at 1:50 for 1 hour at RT. Secondary Antibody: Alexa Fluor 488 Goat Anti-Mouse (green) at 1:100 for 1 hour at RT. Counterstain: DAPI (blue) nuclear stain. Magnification: 63x.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PP5 Monoclonal Antibody, Clone 12F7. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X.

Western Blot analysis of Human A431, HEK293, and Jurkat cell lysates showing detection of ~58 kDa PP5 protein using Mouse Anti-PP5 Monoclonal Antibody, Clone 12F7. Lane 1: MW Ladder. Lane 2: Human A431 (15 μg). Lane 3: Human HEK293 (15 μg). Lane 4: Human Jurkat (15 μg). Load: 15 μg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody at 1:500 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~58 kDa.

Quick Database Links

Gene Symbol

PP5

UniProt

P53041

NCBI Gene ID

5536

RefSeq (Protein):NP_006238.1