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POR Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human POR (NP_000932.3). |
| Target | POR |
| Molecular Weight | Observed: 77kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−POR Rabbit Polyclonal Antibody [orb579142]
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Rabbit
Polyclonal
Unconjugated
100 μlCytochrome P450 Reductase/POR Rabbit Polyclonal Antibody [orb316599]
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100 μgHuman Cytochrome P450 Reductase (CPR) ELISA Kit [orb777363]
Human
0.16-10 ng/mL
0.058 ng/mL
96 T, 48 T

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Western blot analysis of extracts of various cell lines, using POR antibody (orb1274413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Western blot analysis of extracts from normal (control) and POR knockout (KO) HeLa cells, using POR antibody (orb1274413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human esophagus using POR antibody (orb1274413) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using POR Polyclonal Antibody (orb1274413) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using POR Polyclonal Antibody (orb1274413) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using POR Polyclonal Antibody (orb1274413) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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POR Antibody (orb1274413)
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