PON2 Antibody

SKU: orb1249175

Description

PON2 Antibody

Research Area

Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Canine, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa double band observed in lysates of HepG2 (calculated MW of 39.4kDa according to NP_000296.2 and of 38.0kDa according to NP_001018171.1). Recommended concentration: 0.2-0.6µg/mL. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 3.75µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-EKPRARELRIS
• Target: PON2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PON2, paraoxonase 2, A-esterase 2, aromatic esterase 2, paraoxonase nirs, serum aryldialkylphosphatase 2, serum paraoxonase/arylesterase 2

orb1249175 (0.2 ug/ml) staining of HepG2 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249175 (3.75 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

PON2

UniProt

Q15165

RefSeq (Protein):NP_001018171.1, NP_000296.2