POMC Antibody

• Catalog Number: orb1248175
• Category: Antibodies
• Description: POMC Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-NAIIKNAYKKGE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 27kDa band observed in lysates of cell line NIH3T3 and approx 30kDa in Rat Brain lysates (calculated MW of 26.7kDa according to Mouse NP_001265510.1 1 and 26.6kDa according to Rat NP_647542.1). Recommended concentration: 0.3-2µg/ml. Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Human Anterior Pituitary Gland shows vesilulate staining in glandular cells. Recommended concentration: 2-4µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: POMC
• UniProt ID: P01189
• NCBI: NP_000930.1, NP_001030333.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: POMC, proopiomelanocortin, Precursor of MSH, POC,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 27kDa band observed in lysates of cell line NIH3T3 and approx 30kDa in Rat Brain lysates (calculated MW of 26.7kDa according to Mouse NP_001265510.1 1 and 26.6kDa according to Rat NP_647542.1). Recommended concentration: 0.3-2µg/ml. Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Human Anterior Pituitary Gland shows vesilulate staining in glandular cells. Recommended concentration: 2-4µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248175 (0.3 μg/ml) staining of NIH3T3 cell lysate (A) and (2 ug/ml) Rat Brain (B) lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

orb1248175 (2 ug/ml) staining of paraffin embedded Human Pituitary Gland. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.