Polygalacin D

SKU: orb1684305

Description

Polygalacin D is a bioactive small molecule with demonstrated anti-proliferative, anti-inflammatory, and hepatoprotective properties. It effectively inhibits LPS-induced expression of iNOS, COX-2, and PGE2 in RAW 264.7 macrophages without cytotoxicity, supporting its research use in inflammation and liver disease models both in vitro and in vivo.

Research Area

Cell Biology

Key Properties

• CAS Number: 66663-91-0
• MW: 1209.4
• Purity: ≥98% (May vary between batches)
• Formula: C₅₇H₉₂O₂₇
• SMILES: [H][C@@]1(O[C@H]2[C@H](O)CO[C@@]([H])(O[C@H]3[C@H](C)O[C@@]([H])(O[C@@H]4[C@@H](O)[C@@H](O)CO[C@H]4OC(=O)[C@]45CCC(C)(C)C[C@@]4([H])C4=CC[C@]6([H])[C@@]7(C)C[C@H](O)[C@H](O[C@]8([H])O[C@H](CO)[C@@H](O)[C@H](O)[C@H]8O)[C@](C)(CO)[C@]7([H])CC[C@@]6(C)[C@]4(C)C[C@H]5O)[C@H](O)[C@@H]3O)[C@@H]2O)OC[C@](O)(CO)[C@H]1O
• Target: IAP|||Apoptosis
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (1.65 mM);DMSO:55 mg/mL (45.48 mM)

Bioactivity

• In Vitro:
  In this study, we investigated the effects of seven platycodin saponins on the activities of inducible nitric oxide synthase (iNOS) and cyclooxygenase II (COX-2) in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. We found that 2'-O-acetyl Polygalacin D (S1), platycodin A (S2), platycodin D (S3), and Polygalacin D (S6) inhibited LPS-induced NO production in a concentration-dependent manner. Furthermore, these compounds inhibited the expression of LPS-induced iNOS and COX-2 protein and mRNA without an appreciable cytotoxic effect on RAW 264.7 macrophages, and could suppress induction by LPS of pro-inflammatory cytokines such as prostaglandin E2 (PGE2). Treatment with these compounds of RAW 264.7 cells transfected with a reporter construct indicated a reduced level of LPS-induced nuclear factor-kappaB (NF-kappaB) activity and effectively lowered NF-kappaB binding as measured by electrophoretic mobility shift assay (EMSA). The suppression of NF-kappaB activation appears to occur through the prevention of inhibitor kappaB (IkappaB) degradation. In vivo, platycodin saponin mixture (PS) and S3 protected mice from the lethal effects of LPS. The 89% lethality induced by LPS/galactosamine was reduced to 60% and 50% when PS and S3, respectively, were administered simultaneously with LPS
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apoptosis, COX, Inhibitor, NOS, inhibit, IAP, Polygalacin D, PGE

Compound Identifiers

PubChem CID

46173909