Polygalacin D

SKU: orb1217752

Description

Polygalacin D shows anti- proliferation, anti-inflammary, and hepatoprotective activities, it can inhibit the expression of lipopolysaccharide (LPS)-induced iNOS and COX-2 protein and mRNA without an appreciable cytotoxic effect on RAW 264.7 macrophages, and can suppress induction by LPS of pro-inflammatory cytokines such as prostaglandin E2 (PGE2).

Key Properties

• CAS Number: 66663-91-0
• MW: 1209.4
• Purity: >98% (HPLC)
• Formula: C₅₇H₉₂O₂₇
• SMILES: O[C@H]([C@@H]([C@@H](O[C@@]1([H])[C@@H]([C@H]([C@H](O)CO1)O[C@@]2([H])[C@@H]([C@](CO)(O)CO2)O)O)[C@H](C)O3)O)[C@]3([H])O[C@H]([C@H]([C@@H](O)CO4)O)[C@@H]4OC([C@]56[C@](CC(C)(C)CC6)([H])C7=CC[C@@]([C@@]8([C@@]([C@](C)([C@@H](O[C@]9([H])O[C@@H]([C@@H](O)[C@H](O)[C@H]9O)CO)[C@@H](O)C8)CO)([H])CC%10)C)([H])[C@]%10(C)[C@]7(C)C[C@H]5O)=O
• Target: Apoptosis
• Solubility: In Vitro: DMSO : 100 mg/mL (82.69 mM)

Bioactivity

• In Vitro:
  Polygalacin D (0-40 μM; 48 hours) inhibits cell proliferation with IC50s of26.49 μM in the A549 cells and 20.52 μM in the H460 cells. Polygalacin D (0-20 μM; 48 hours) increases the proportion of early and late apoptotic cells, and nuclear condensation is observed in A549 and H460 cells. Polygalacin D (0-20 μM; 48 hours) may exerts its apoptotic effects by regulating the apoptotic proteins and the IAP family of proteins in A549 and H460 cells. Cell Proliferation Assay Cell line: A549 and H460 cancer cell lines. Concentration: 10 μM, 20 μM, 40 μM. Incubation time: 48 hours. Result: Inhibited the proliferation of NSCLC cell lines. Apoptosis Analysis Cell line: A549 and H460 cancer cell lines. Concentration: 10 μM, 20 μM. Incubation time: 48 hours. Result: Induced apoptosis of NSCLC cell lines. Western blot analysis. Cell line: A549 and H460 cancer cell lines. Concentration: 10 μM, 20 μM. Incubation time: 48 hours. Result: Decreased survivin, c-IAP-1 and c-IAP-2 expression.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Apoptosis