POLR2D Antibody

SKU: orb1258417

Description

POLR2D Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IF, IP, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:10 - 1:100IP: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human POLR2D (NP_004796.1).
• Target: POLR2D
• Molecular Weight: Observed: 17kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA-directed RNA polymerase II subunit RPB4, RNA polymerase II subunit B4, DNA-directed RNA polymerase II subunit D, RNA polymerase II 16 kDa subunit, RPB16, POLR2D

Western blot analysis of extracts of various cell lines, using POLR2D antibody (orb1258417) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of A549 cells using POLR2D antibody (orb1258417).

Immunoprecipitation analysis of 200 ug extracts of HepG2 cells using 1 ug POLR2D antibody (orb1258417). Western blot was performed from the immunoprecipitate using POLR2D antibody (orb1258417) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

POLR2D

UniProt

O15514