POLR2D Antibody

• Catalog Number: orb1258417
• Category: Antibodies
• Description: POLR2D Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human POLR2D (NP_004796.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:10 - 1:100IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 17kDa
• Target: POLR2D
• UniProt ID: O15514
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: DNA-directed RNA polymerase II subunit RPB4, RNA p
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:10 - 1:100IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using POLR2D antibody (orb1258417) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of A549 cells using POLR2D antibody (orb1258417).

Immunoprecipitation analysis of 200 ug extracts of HepG2 cells using 1 ug POLR2D antibody (orb1258417). Western blot was performed from the immunoprecipitate using POLR2D antibody (orb1258417) at a dilution of 1:1000.