POLR2C Antibody

SKU: orb1258524

Description

POLR2C Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IP, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1:500 - 1:2000IP: 1:20 - 1:50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human POLR2C (NP_116558.1).
• Target: POLR2C
• Molecular Weight: Observed: 38kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RPB3, RPB31, hRPB33, hsRPB3, DNA-directed RNA polymerase II subunit RPB3, DNA-directed RNA polymerase II 33 kDa polypeptide, DNA-directed RNA polymerase II subunit C, RNA polymerase II subunit 3, RNA polymerase II subunit B3, RPB33, polymerase (RNA) II (DNA directed) polypeptide C, 33kDa, polymerase (RNA) II subunit C

Western blot analysis of extracts of various cell lines, using POLR2C antibody (orb1258524) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunoprecipitation analysis of 150 ug extracts of SW620 cells using 3 ug POLR2C antibody (orb1258524). Western blot was performed from the immunoprecipitate using POLR2C antibody (orb1258524) at a dilution of 1:500.

Quick Database Links

Gene Symbol

POLR2C

UniProt

P19387