POLR2C Antibody

• Catalog Number: orb1258524
• Category: Antibodies
• Description: POLR2C Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human POLR2C (NP_116558.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 38kDa
• Target: POLR2C
• UniProt ID: P19387
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: RPB3, RPB31, hRPB33, hsRPB3, DNA-directed RNA poly
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using POLR2C antibody (orb1258524) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunoprecipitation analysis of 150 ug extracts of SW620 cells using 3 ug POLR2C antibody (orb1258524). Western blot was performed from the immunoprecipitate using POLR2C antibody (orb1258524) at a dilution of 1:500.