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POLR2B Antibody
SKU: orb1257247
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2B (NP_000929.1). |
| Target | POLR2B |
| Molecular Weight | Observed: 134kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−POLR2B, RPB2, POL2RB, hsRPB2, hRPB140
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Western blot analysis of extracts of various cell lines, using POLR2B antibody (orb1257247) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat liver using POLR2B antibody (orb1257247) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using POLR2B antibody (orb1257247) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using POLR2B antibody (orb1257247) at dilution of 1:100 (40x lens).
Quick Database Links
Gene Symbol
POLR2B
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
POLR2B Antibody (orb1257247)
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