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POLR2A Antibody
SKU: orb1261289
Featured
Description
Research Area
Neuroscience
Images & Validation
−Item 1 of 6
| Tested Applications | ChIP, IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Porcine, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2A (NP_000928.1). |
| Target | POLR2A |
| Molecular Weight | Observed: 250kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−POLR2A, HRPB220, HsRPB1, POLR2, RpIILS, RPOL2, RNA Polymerase II, RPO2, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1
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Western blot analysis of extracts of various cell lines, using POLR2A antibody (orb1261289) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat testis using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).
Quick Database Links
Gene Symbol
POLR2A
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
ChIP
Chromatin Immunoprecipitation
POLR2A Antibody (orb1261289)
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