POLR2A Antibody

• Catalog Number: orb1261289
• Category: Antibodies
• Description: POLR2A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2A (NP_000928.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50ChIP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 250kDa
• Target: POLR2A
• UniProt ID: P24928
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: POLR2A, HRPB220, HsRPB1, POLR2, RpIILS, RPOL2, RNA
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50ChIP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using POLR2A antibody (orb1261289) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat testis using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat pancreas using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).