POLR1C Antibody (C-term)

• Catalog Number: orb1931409
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB17524
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 39250 Da
• Target: This POLR1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-346 amino acids from the C-terminal region of human POLR1C.
• UniProt ID: O15160
• NCBI: NP_976035.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: DNA-directed RNA polymerases I and III subunit RPA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with POLR1C antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-POLR1C Antibody (C-term) at 1:2000 dilution. Lane 1: K562 whole cell lysate. Lane 2: SW480 whole cell lysate. Lane 3: MDA-MB-231 whole cell lysate. Lane 4: A2058 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: HepG2 whole cell lysate. Lane 7: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 39 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.