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Item 1 of 5
POLG Antibody (C-term)
Catalog Number: orb1935340
| Catalog Number | orb1935340 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB34378 |
| Tested applications | FC, IF, IHC-P, WB |
| Predicted Reactivity | Rat |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:10~50, WB: 1:500, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 139562 Da |
| Target | This POLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1120-1148 amino acids from the C-terminal region of human POLG. |
| UniProt ID | P54098 |
| NCBI | NP_002684.1, NP_001119603.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | DNA polymerase subunit gamma-1, Mitochondrial DNA Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
POLG Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35 ug/lane). This demonstrates the POLG antibody detected the POLG protein (arrow).
Confocal immunofluorescent analysis of POLG Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
POLG Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Anti-POLG Antibody (C-term) at 1:500 dilution + HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 140 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
POLG Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of POLG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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