POLDIP2 Antibody

SKU: orb1248989

Description

POLDIP2 Antibody

Research Area

Cancer Biology, Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 37kDa band observed in lysates of cell lines K562 and MCF7 and of Mouse Liver (calculated MW of 42.0kDa according to NP_056399.1). Approx 37kDa band observed in Rat Vascular Smooth Muscle Cells lysates. The band dimished upon siRNA knock down. Recommended concentration: 1-3µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: KTHTYYQVLIDARDC
• Target: POLDIP2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

POLDIP2, polymerase (DNA-directed), delta interacting protein 2, DKFZp586F1524, PDIP38, POLD4, 38 kDa DNA polymerase delta interaction protein, p38, polymerase delta interacting protein 38, polymerase delta-interacting protein 2

orb1248989 (1 ug/ml) staining of K562 (A), MCF7 (B) and Mouse Liver (C) lysates (35 ug protein in RIPA buffer). Primary incubation was overnight at 4C. Detected by chemiluminescence.

orb1248989 (0.5 ug/ml) staining of Rat VSMC lysate (35 ug protein in RIPA buffer). Primary incubation was overnight at 4C. Detected by chemiluminescence.

orb1248989 (2 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was overnight at 4C. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

POLDIP2

UniProt

Q9Y2S7

RefSeq (Protein):NP_056399.1