POLDIP2 Antibody

• Catalog Number: orb1248989
• Category: Antibodies
• Description: POLDIP2 Antibody
• Target: POLDIP2
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: KTHTYYQVLIDARDC
• UniProt ID: Q9Y2S7
• Tested applications: ELISA, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 37kDa band observed in lysates of cell lines K562 and MCF7 and of Mouse Liver (calculated MW of 42.0kDa according to NP_056399.1). Approx 37kDa band observed in Rat Vascular Smooth Muscle Cells lysates. The band dimished upon siRNA knock down. Recommended concentration: 1-3ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: POLDIP2, polymerase (DNA-directed), delta interact
• Research Area: Cancer Biology, Cell Biology
• Note: For research use only
• NCBI: NP_056399.1

orb1248989 (1 ug/ml) staining of K562 (A), MCF7 (B) and Mouse Liver (C) lysates (35 ug protein in RIPA buffer). Primary incubation was overnight at 4C. Detected by chemiluminescence.

orb1248989 (0.5 ug/ml) staining of Rat VSMC lysate (35 ug protein in RIPA buffer). Primary incubation was overnight at 4C. Detected by chemiluminescence.

orb1248989 (2 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was overnight at 4C. Detected by chemiluminescence.